Proteomics: Principles and Techniques by Sanjeeva Srivastava (IIT Bombay)

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source: nptelhrd    2013年6月5日
Biotechnology - Proteomics: Principles and Techniques by Prof. Sanjeeva Srivastava, Department of Biotechnology, IIT Bombay. For more details on NPTEL visit http://nptel.iitm.ac.in

01 Introduction to Proteomics Course 1:03:26
02 Central Dogma: Basics of DNA, RNA, Proteins 48:57
03 Genomics and Transcriptomics: Why proteomics? 45:42
04 Proteins: Amino acids and structural levels of proteins 52:53
05 Proteins: Folding and misfolding 47:45
06 Protein Purification and Peptide Isolation using Chromatography 38:39
07 Enzymes: Basic concepts, Catalytic and Regulatory strategies 56:10
08 Proteomics and Systems Biology 1:12:45
09 Sample preparation for proteomics applications 48:52
10 Sample preparation for proteomics applications: Serum and bacterial proteome 55:24
11 Sample preparation for proteomics applications 51:26
12 Gelbased Proteomics 55:39
13 Gel-based Proteomics Two-dimensional electrophoresis(continued) 49:05
14 Gel-based Proteomics Two-dimensional electrophoresis Workflow 57:36
15 Two-dimensional electrophoresis: Image processing and data analysis 1:05:57
16 Two-dimensional difference gel electrophoresis (2D DIGE) 45:19
17 Difference gel electrophoresis (continued) Discussion and data analysis 45:14
18 Applications of two dimensional electrophoresis 44:34
19 Applications of 2-DE and DIGE 45:24
20 Fundamentals of mass spectrometry 47:05
21 Matrix assisted laser desorption/ionization-Time of Flight (MALDI-TOF) 55:01
22 Liquid chromatography-Mass spectrometry (LC-MS/MS) 51:41
23 Hybrid-MS/MS Configurations 41:27
24 Quantitative Proteomics: Stable Isotope Labeling by Amino Acids in Cell Culture(SILAC) 50:56
25 Quantitative Proteomics: iTRAQ and TMT 43:49
26 Interactomics: Yeast Two-Hybrid Immunoprecipitation Protein microarrays 53:11
27 Microarray workflow: Label-based detection techniques 44:20
28 Microarray related concepts: Recombinational cloning Cell-free expression 56:11
29 Cell-free synthesis based protein microarrays 56:23
30 Generating Protein Microarrays: Focus on Nucleic Acid Programmable Protein Array 59:14
31 Microarray work-flow: Image scanning and processing 55:48
32 Microarray work-flow: Data analysis 1:02:11
33 Applications of protein microarrays 53:13
34 Applications of cell free protein microarrays 59:10
35 Label-free techniques: SPR and SPRi 48:25
36 Label-free techniques: SPRi, Ellipsometry, Interference 41:34
37 Surface Plasmon Resonance: Biacore SPR and data analysis 43:36
38 Nanotechniques in proteomics 52:09
39 Detection System: Diffraction-based biosensors 52:57
40 Proteomics: Advances and Challenges 1:07:38